Fig. 6.

Quantification of expression of ALPL mRNA (A–E) and TNSALP activity (F–J) in placentomes of ewes treated with either corn oil (CO) or progesterone (P4). Representative images of cell-specific localization of TNSALP protein and activity in placentomes (K). Rabbit IgG (RIgG) controls were included at a protein concentration equivalent to that for the TNSALP antibody as a negative control for the immunohistochemical staining. Mean values presented ± SEM. Scale bars represent 100 µm. Car = caruncle, Cot = cotyledon, BV = blood vessel. Ewes were euthanized and then hysterectomized on either Day 9 (CO, n = 5; P4, n = 6), 12 (CO, n = 9; P4, n = 4), or 125 (n = 5 CO singles, 9 CO twins, 5 P4 singles, and 5 P4 twins) of gestation