Fig. 4.

TonEBP regulates the expression and activity of aurora A kinase. a. RPE1 cells were serum-starved for 24 h, transfected with TonEBP siRNA for 24 h, followed by serum stimulation, and harvested at different time points, as indicated; b. Cells were transfected with siRNAs against TonEBP for 24 h, followed by 24 h of serum starvation, and immunostained for ARL13b (green) and γ-tubulin (red). Scale bar, 25 μm; c. Quantification of ciliated cell percentage as shown in (B). Data is represented as mean ± SD (n = 3 experiments). Two hundred cells were scored per condition per experiment; **P < 0.01, ***P < 0.001, Student’s t-test; d. Cells were transfected with TonEBP siRNA for 24 h, serum-starved, followed by serum restimulation. Then, cells were harvested at different time points and subjected to Western blot and qPCR. qPCR data is represented as mean ± SD (n = 3 experiments). ***P < 0.001, Student’s t-test; e. Cells were transfected with TonEBP-mCherry plasmid for 24 h, followed by serum starvation, and subjected to Western blot and qPCR. qPCR data is represented as mean ± SD (n = 3 experiments). *P < 0.05, Student’s t-test