TABLE 3.
I-309 and SDF-1α inhibit infection of thymocytes with HIV-1NL4-3 in a dose-dependent mannera
| Day after infection | Chemokine | Dose (μg/ml) | Mean p24 concn (pg/ml) ± SEM | % Inhibition |
|---|---|---|---|---|
| 5 | None | 1,038.7 ± 18.7 | ||
| I-309 | 2.0 | 629.8 ± 16.7 | 40 | |
| 0.2 | 770.3 ± 2.8 | 26 | ||
| SDF-1α | 2.0 | 287.9 ± 1.1 | 72 | |
| 0.2 | 529.8 ± 27 | 49 | ||
| I-309 + SDF-1α | 1.0 + 1.0 | 76.9 ± 20.8 | 93 | |
| MCP3 | 2.0 | 1,421 ± 133.1 | 0 | |
| 7 | None | 9,847.5 ± 627.6 | ||
| I-309 | 2.0 | 4,541.2 ± 53.0 | 54 | |
| 0.2 | 8,578.7 ± 141.4 | 13 | ||
| SDF-1α | 2.0 | 1,841.2 ± 0 | 81 | |
| 0.2 | 6,185 ± 97.2 | 37 | ||
| I-309 + SDF-1α | 1.0 + 1.0 | 241.2 ± 229.7 | 98 |
a
All chemokines were added to CD4 SP thymocytes 1 to 2 h prior to infection and then maintained at the indicated concentrations during culture. Thymocytes were infected with HIV-1NL4-3 at an MOI of 0.01; supernatants were harvested at the indicated times and analyzed for p24 by ELISA. Chemokine-mediated inhibition of p24 production was calculated by comparison with control cultures without chemokines.