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. 2000 Aug;74(15):6964–6974. doi: 10.1128/jvi.74.15.6964-6974.2000

FIG. 4.

FIG. 4

Comparison of the in vitro growth properties of several recombinant MHV-68 mutants and WT MHV-68. BHK-21 cells were infected at an MOI of 0.1. Cells and supernatants were harvested at the indicated time points, and viral titers were determined by plaque assay. Titers at 0 h represent input inocula. (A) Growth properties of RγHV68A98.01 compared to WT MHV-68; (B) Expression of gfp in NIH3T3 cells infected with RγHV68A98.01; (C) Growth properties of RγHV68A98.02 compared to RγHV68A98.01; (D) Lack of gfp expression in NIH3T3 cells infected with RγHV68A98.02; (E) Growth properties of RγHV68A98.03 and RγHV68A98.04 compared to RγHV68A98.01; (F) Southern blot analysis of EcoRI-digested DNA isolated from cells infected with WT MHV-68 (lane 1), RγHV68A98.01 (lane 2), RγHV68A98.03 (lane 3), and RγHV68A98.04 (lane 4) with a probe specific for the EcoRI K fragment after digestion of the DNA with EcoRI.