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. 2024 Jul 3;28(13):e18516. doi: 10.1111/jcmm.18516

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© 2024 The Author(s). Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Decoding tumour cell cluster dynamics. (A) Enrichment analysis across distinct tumour cell clusters using Gene Set Variation Analysis (GSVA), visualized through a heatmap. (B) Expression dynamics across pseudotime are depicted in a heatmap, showcasing gene expression intensity variations. (C) Developmental trajectories of various tumour cell clusters are illustrated via pseudotime analysis, with cells colour‐coded based on tumour clusters or progression through pseudotime. (D) Gene ontology (GO) enrichment analysis identifies and highlights enriched pathways in genes from Clusters 1 and 2 as shown in (B), covering aspects of biological process (BP), cellular component (CC) and molecular function (MF).