Table 1.

GPCR	Expression system	Isotope labeling	Membrane Mimetic	Ref.
A2AAR	Yeast (P. pastoris)	ε−13CH3-Ile, 2H	Detergent micelles	[6]
		u-15N, ~70%2H	Detergent micelles	[7], [8], [9]
		ε−13CH3-Met	Lipid nanodiscs	[10]
α1AAR	E.coli	ε−13CH3-Met	Detergent micelles	[11]
β1AR	Insect cells (Sf9)	15N-Valine	Detergent micelles	[12] , [13], [14]
		ε−13CH3-Met	Detergent micelles	[15]
	Insect cells (Sf9)	u-15N, >60%2H	Detergent micelles	[16]
	Mammalian cells	ε−13CH3-Met	Detergent micelles	[17]
β2AR	Insect cells (Sf9)	(13CH3)-Lys reductive dimethylation	Detergent micelles	[18], [19]
		ε−13CH3-Met	Detergent micelles	[20,21]
		ε−13CH3-Met, 2H	Lipid nanodiscs	[22]
		β−13CH3-Ala, 2H	Detergents micelles Lipid nanodiscs	[23]
	Insect cells (Sf9) and E.coli	ε−13CH3-Met, 2H, c-term-2H, 13C, 15N	Lipid nanodiscs	[24]
		[2,3,3-2H, 15N]-leucine	Detergent micelles	[25]
BLT2	E.coli	ε−13CH3-Met and ε−13CH3-Ile	Lipid nanodiscs	[26]
CB2	Yeast (P. pastoris)	ε−13CH3-Ile	Detergent micelles	[27]
ACKR3	Insect cells	ε−13CH3-Met	Detergent micelles	[28]
H1R	Yeast (P. pastoris)	u-15N, ~70%2H	Detergent micelles	[29]
M2R	Insect cells (Sf9)	ε−13CH3-Met	Detergent micelles	[30]
NTR1	E.coli	13C-MMTS	Detergent micelles	[31]
		ε−13CH3-Met	Detergent micelles	[32]
μOR	Insect cells	ε−13CH3-Met, 2H	Detergent micelles	[33]
		(13CH3)-Lys reductive dimethylation	Detergent micelles	[34], [35]
OX2R	Yeast (P. pastoris)	ε−13CH3-Ile	Detergent micelles	[36]
CB1	Yeast (P. pastoris)	ε−13CH3-Ile	Detergent micelles	[36]
Rhodopsin	Mammalian cells	α,ε−15N-Trp	Detergent micelles	[37],[38]
		13Cβ-Ser, 13Cβ-Cys, 13Cα-Gly		[39]

Abbreviations: A_2AAR, adenosine A_2A receptor; α_1AAR, α₁A-adrenergic receptor; β₁AR, β₁-Adrenergic receptor; β₂AR, β₂-Adrenergic receptor; BLT₂, leukotriene B₄ receptor 2; CB2, Cannabinoid receptor type 2; ACKR3, atypical chemokine receptor 3; H₁R, histamine H₁ receptor; M₂R, muscarinic acetylcholine receptor M2; NTR1, neurotensin receptor type 1; μOR, μ-opioid receptor; OX₂R, orexin receptor type 2; CB1, cannabinoid receptor type 1.