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. 2024 May 25;11(6):nwae182. doi: 10.1093/nsr/nwae182

Figure 4.

Graphs and data on IM-MS assay of α-syn in lipid environments with sub-figures labeled from a to e, showing the IM-MS schematics, IM-MS spectrum, IM-MS spectrum deconvolution, the comparison of α-syn conformers distribution with LPC vs. DPPC and activation-induced unfolding of α-syn with or without LPC.

LPC compacts α-syn and increases its stability. (a) The schematics of IM-MS technology. (b) IM-MS spectrum of α-syn with charge-state distributions. Monomeric charged state (11+) is highlighted with a frame, and is zoomed in with labels in the inset. (c) IM-MS spectrum isolated from 11+ charged α-syn was deconvolved into four conformers (A–D) on the basis of Ω acquired from α-syn in the presence of LPC. (d) Population distribution change of the four conformers (A–D) upon the addition of LPC (upper) and DPPC (lower). (e) The compact conformer A of α-syn is stabilized by LPC and resists activation-induced unfolding.