Fig 5. Representative images for lectin histochemistry of renal tubules and collecting ducts (CDs) in cats and dogs.
(A) Soybean agglutinin Glycine max (soybean) seeds (SBA). In cats, positive reactions are observed in the apical portion of epithelial cells in the distal straight tubules (DSTs) and CDs. In dogs, these reactions are weak in DSTs; furthermore, several CD epithelial cells show SBA-positive reactions in the cytoplasm (arrowheads), though there are several negative cells. (B) Griffonia simplicifolia Ⅱ (GSL-Ⅱ) and Phaseolus vulgaris leukoagglutinin (PHA-L). Positive reactions for GSL-II and PHA-L are observed in the apical portion of the CD epithelium at the medulla but not in the cortex of cats and dogs, respectively. Bars = 50 μm.