Table 1. The mass spectrometry analysis on the exonuclease Ⅲ (ExoIII)-treated ssDNA oligos.
| Sample(Theoretical mass) | ssDNA without ExoIII | ssDNA treated with ExoIII | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Mass peak (Da) | Intensity | Relative (%) | Presumed products (Theoretical mass) | Mass peak (Da) | Intensity | Relative (%) | Presumed products (Theoretical mass) | Structure of the top intensity | |
| Probe 1 (7504) | 7501.1 | 4.79E+07 | 100 | FITC-CAAACCCAGAGCCAATCTTATCT (7504) | 2914.9 | 6.31E+06 | 100 | FITC-CAAACCCA (2915.6) |
|
| 7523.4 | 1.69E+07 | 35.28 | FITC-CAAACCCAGAGCCAATCTTATCT (7504) + Na+ | 2601.5 | 4.76E+06 | 75.37 | FITC-CAAACCC (2602.4) | ||
| 7546.1 | 1.02E+07 | 21.4 | FITC-CAAACCCAGAGCCAATCTTATCT (7504)+2Na+ | 4047.7 | 1.10E+06 | 17.43 | Undefined | ||
| 7567.9 | 3.45E+06 | 7.2 | FITC-CAAACCCAGAGCCAATCTTATCT (7504)+3Na+ | 2312.2 | 1.07E+06 | 17.01 | Undefined | ||
| 10106.2 | 3.10E+06 | 6.47 | Undefined | 5395.5 | 6.18E+05 | 9.79 | FITC-CAAACCCAGAGCCAAT (5397.2) | ||
| Probe 2 (7063) | 7059.7 | 3.34E+07 | 100 | FAM-GGGTGGGCGGAAAACTATTTC (7063) | 1287.5 | 5.53E+05 | 100 | Mass peak of ExoIII |
|
| 7082.3 | 7.84E+06 | 23.46 | FAM-GGGTGGGCGGAAAACTATTTC (7063)+Na + | 627.8 | 3.35E+05 | 60.59 | Mass peak of ExoIII or TT (P) (626.4) | ||
| 7104.6 | 2.95E+06 | 8.84 | FAM-GGGTGGGCGGAAAACTATTTC (7063)+2Na+ | 13646.1 | 2.01E+05 | 36.26 | Undefined | ||
| 10106.5 | 2.27E+06 | 6.8 | Undefined | 10107.2 | 1.97E+05 | 35.57 | Undefined | ||
| 7341.3 | 1.20E+06 | 3.58 | Undefined | 1979.4 | 1.92E+05 | 34.81 | Undefined | ||
| 13461.6 | 1.07E+006 | 7.94 | Undefined | 4639.6 | 1.59E+05 | 28.74 | FAM-GGGTGGGCGGAAA (4641.6) | ||
| 11549.0 | 6.24E+005 | 6.55 | Undefined | 6655.3 | 1.55E+05 | 28.11 | Undefined | ||
| 3376.6 | 4.57E+005 | 2.88 | Undefined | 1291.6 | 1.45E+05 | 26.29 | Mass peak of ExoIII or GCGG/GGCG/GGGC (P) (1293.8) | ||
| 7481.5 | 3.82E+005 | 5.67 | Undefined | 9126.5 | 1.02E+05 | 18.42 | Undefined | ||
| 957.7 | 1.02E+05 | 18.39 | AAA (P) (957.6) | ||||||
| Probe 3 (6658) | 6655.2 | 3.30E+07 | 100 | FAM-AGTCCGT TTGTTCTTGTGGC (6658) |
2936.9 | 8.36E+06 | 100 | FAM-AGTCCGTT (2937.6) |
|
| 6677.5 | 1.06E+07 | 32.18 | FAM-AGTCCGTTTGTTCTTGTGGC (6658) + Na+ | 3240.9 | 2.77E+06 | 33.1 | FAM-AGTCCGTTT (3241.8) | ||
| 6699.9 | 6.00E+06 | 18.15 | FAM-AGTCCGTTTGTTCTTGTGGC (6658)+2Na+ | 2632.7 | 2.34E+06 | 27.99 | FAM-AGTCCGT (2633.4) | ||
| 6721.6 | 1.85E+06 | 5.59 | Undefined | 2328.7 | 8.11E+05 | 9.69 | FAM-AGTCCG (2329.2) | ||
| 6936.6 | 1.29E+06 | 3.91 | Undefined | 643.8 | 4.59E+05 | 5.48 | Undefined | ||
| ExoIII | 1287.6 | 5.44E+05 | 100 | Undefined | |||||
| 627.8 | 3.45E+05 | 63.38 | Undefined | ||||||
| 2639.4 | 1.91E+05 | 35.09 | Undefined | ||||||
| 1291.6 | 1.47E+05 | 27.04 | Undefined | ||||||
| 19174.8 | 1.36E+05 | 25.08 | Undefined | ||||||