TABLE 1.
Reporter plasmids containing HCV IRES sequences with nucleotide substitutions identified in lymphoblastoid cells infected with H77 virus
| Sequence designation
|
Nucleotide substitution at base position:
|
Quasispecies distributiona
|
|||||
|---|---|---|---|---|---|---|---|
| Plasmid | Nakajima et al. (23)b | 107 | 204 | 243 | H77 inoculum | HPBMa10-2 cells | Daudi cells |
| G-C-Gc | NC1 | G | C | G | 7/8 | ||
| G-A-A | NC6 | A | A | 1/8 | |||
| A-A-A | NC7 | A | A | A | 12/12 | 10/11 | |
| A-C-A | NC9 | A | A | 1/11 | |||
| G-A-G | A | ||||||
| G-C-A | A | ||||||
| A-C-G | A | ||||||
| A-A-G | A | A | |||||
a
Number of clones with the indicated sequence/number of all clones recovered from the source (23). HPBMa10-2 cells were infected for 193 days and Daudi cells were infected for 308 days prior to RT-PCR amplification and isolation of sequences.
b
Designation of quasispecies sequence identified in H77 inoculum or in virus recovered from infected lymphoblastoid cells by Nakajima et al. (23).
c
Dominant wild-type virus quasispecies in H77 inoculum.