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. Author manuscript; available in PMC: 2024 Jul 3.
Published in final edited form as: Cell Host Microbe. 2023 Oct 30;31(11):1850–1865.e5. doi: 10.1016/j.chom.2023.10.004

Figure 1. DENV1/3 E glycoprotein recombinant viruses.

Figure 1.

(A) Design of DENV1/3 glycoproteins. Structural glycoprotein design. DENV1/3 EDI, EDII, and EDIII chimeras are depicted in a hexameric raft formation with 6 antiparallel E glycoprotein monomers forming 3 parallel dimers (PDB: 3J27, for visualization); DENV1 = dark blue, DENV3 = pink, DENV1 1F4/14C10 epitopes =cyan on the EDII chimera and residue mutations in the EDIII chimera = lime green. The premembrane (prM) protein is pictured to the right of the EDII and EDIII chimeras (PDB: 6IDI, for visualization). For prM, DENV1= black, DENV3 = teal.

(B) DENV1/3 particle symmetry. DENV3 E protein 3-fold, 2-fold, and 5-fold symmetry are represented on the whole virion with DENV1/3 EDI, DENV1/3 EDII, and DENV1/3 EDIII respectively, with DENV3 = pink, DENV1 = dark blue (PDB: 3J6S_*60, for visualization).

(c) Primary sequence variation in chimeric viruses. E glycoprotein DENV3 original encoded changes into DENV1 are represented for DENV1/3 EDII and EDIII chimeras, DENV3 = pink, DENV1 = dark blue.