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. Author manuscript; available in PMC: 2024 Jul 3.
Published in final edited form as: Cell Host Microbe. 2023 Oct 30;31(11):1850–1865.e5. doi: 10.1016/j.chom.2023.10.004

Figure 4. DENV3 hmAbs-115 and −419 target clustered residues in EDII.

Figure 4.

(A) DENV3 EDII epitope mapping strategy. DENV3/3 EDII, G-III into G-IV parent (9 residues) and 4 DENV3/3, G-III into G-IV cluster chimeras (C1–C4) encoding between 2 and 6 DENV3 G-III residues are depicted, G-III changed residues = blue spheres (PDB: 3J27, for visualization). C2 and C3 together make the residues encoded into C4.

(B) hmAB neutralization phenotypes. DENV1/3 EDII-targeting mAbs-115 and −419 neutralize C3 and C4, with the critical residues lying in C3.

(C) Annotated location of nAb epitopes in EDII. The critical mAb-115 and −419 epitope residues are depicted on the whole viron and are located at the EDII-EDII dimer interface (E62, G63, Q120, P124) (PDB: 3J6S_*60, for visualization).