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a) Cartoon depiction of GPR161 stabilization, solubilization, and purification. b) Size-exclusion chromatogram (left) and SDS-PAGE gel (right) of purified GPR161-Gs complex with Nb35. Purification and SDS-PAGE gel were done once and not repeated. Chromatogram and SDS-PAGE gel are shown for preparation used for cryo-EM analysis.
