Extended Data Fig. 8. Photolabeling with LKM238 and mass spectrometry sequence coverage.

a) Product ion spectrum of LKM238-labeled GPR161-miniG_s with peptides mapped to TM6. This peptide is modified with a mass consistent with LKM238 at position K267^6.32. Red brackets and peaks indicate product ions that contain the LKM238 adduct. b) Mass spectrometric sequence coverage of GPR161-miniG_s. Underlined segments indicate transmembrane spanning helices, red font indicates peptides identified by tandem MS analysis and gray font indicates glycosylation sites.