Figure 1.

(a) The ratio of high-quality mapped reads between male and female samples meeting our quality criteria (4% FF, 1.5 M of reads). A significant cluster of female samples approaches a value of 0, with most positioned below the 0.01 threshold, emphasizing the method's accuracy. (b) The analysis of the seven specific genes on chromosome Y. Based on this analysis, the majority of samples accurately determined sex, with a small percentage inaccurately predicted. This highlights the precision and effectiveness of our pipeline's methodologies in fetal sex determination.