FIG. 5.

Low LCMV-induced Ly49G2⁺ CTL-mediated lysis of allogeneic (H2^d) P815 and GP276-pulsed syngeneic (H2^b) targets, and failure to rescue allospecific killing with anti-Ly49G2 MAb. Cultured CD8⁺ Ly49G2⁻ (G2⁻) and CD8⁺ Ly49G2⁺ (G2⁺) cells were used in CTL assays against a variety of targets: LCMV-infected syngeneic (H2^b) MC57G and allogeneic (H2^d) P815 cells (effector-to-target ratios = 2.5:1, 5:1, 10:1, and 20:1) (A); P815 cells in the presence and absence of anti-Ly49G2 MAb (α-G2; 5 μg/well) (B); and syngeneic (H2D^b) L5neo3 cells pulsed with 1 μM LCMV immunodominant peptide GP33, NP396, or GP276 (effector-to-target ratios = 0.31:1, 0.63:1, 1.25:1, and 2.5:1) (C).