Table 2.

Thermocycling program for gene primers.

Bacteria	Target gene	Primary denaturation	Secondary denaturation	Annealing	Extension	No. of cycles	Final extension
P. aeruginosa	rpoB	94°C 3 min	94°C 60sec	58°C 60sec.	72°C 2 min	30	72°C 2 min
	LasB	94°C 5 min	94°C 60 sec	60 °C 1 min	72°C 1 min	35	72°C 7 min
V. parahaemolyticus	Tdh	94°C 5 min	94°C 1 min	55°C 1 min	72°C 1 min	30	72°C 7 min
	ToxR	94°C 10 min	94°C 1 min	63°C 1.5 min	72°C 1.5 min	20	72°C 10 min
A. hydrophila	aerA	94°C 5 min	94°C 30 sec	54°C 30 sec	72°C 1 min	30	72°C 10 min
	Act