FIG. 2.

Plus strand nucleotide sequence of the lacZα gene region in a vpr null mutant HIV-1 vector complemented with Vpr*W54R. The start for nucleotide numbering is the beginning of the 5′ long terminal repeat of the HIV-1 vector. The locations of base substitution, frameshift, and deletion mutations in vpr null mutant HIV-1 vectors from parallel experiments that were complemented with Vpr*W54R or noncomplemented are shown above and below the nucleotide sequence, respectively. The start and stop codons of the lacZα open reading frame and the lac operator sequence are boxed. Nucleotide positions of base pair substitutions (letters above or below the sequence), +1 frameshifts (letters with ▾), −1 frameshifts (▿) and deletions (solid black lines with arrows and adjacent to the deletion names) are indicated. Mutations from the same mutant are designated with a Greek letter adjacent to each mutation; identical Greek letters indicate mutations from the same mutant.