Extended Data Fig. 7. Protective phenotype in OMM12 mice is largely toxin-independent.
Mice were precolonized with WT MK01 or sKO or left untreated before infection with S. Tm. S. Tm burden in the lumen and tissue of (a) cecum, (b) colon, (c) small intestine and (d) various secondary organs including spleen, liver and MLN of infected OMM12 mice at day 6 p.i. (e) Colon length at day 6 p.i. in K. oxytoca colonized mice and control mice. (A-E) The mean ± SEM of n = 3 independent experiments with n = 12 (PBS/sKO) and n = 13 (WT) mice per group. P values indicated represent Ordinary One-Way ANOVA with Dunn’s multiple comparisons test with *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. (f) β-diversity of OMM12 mice using non-metric multidimensional scaling (NMDS). Different groups and sampling time points are indicated. Individual effect size of tested covariates is indicated. To calculate the variance explained by individual factors such as group and time point, a permutational multivariate analysis of variance (ADONIS) was used. A significant effect was dedicated when p < 0.05 and R2 > 0.10 (equivalent to 10 % of explained variance) was observed. (g) Microbiome composition (relative abundance) of each single mouse in different groups (PBS, WT and sKO-colonized) at various time points of the experiment are displayed on species level. (f-g) Data derived from n = 3 independent experiments with n = 14 (PBS), n = 12 sKO, n = 16 (WT) mice.