Table 5.
Effects of sole bacterial inoculation with Bacillus sp. GFB04 and Klebsiella sp. GRB10 on nutrient contents in lettuce shoot under optimum temperature (20/15 °C, d/n) and heat stress (30/25 °C, d/n) conditions
| Shoot | |||||||
|---|---|---|---|---|---|---|---|
| Treatments | Elements (μg/g) | ||||||
| TEMP | INOC | N | P | K | Ca | Mg | Fe |
| Control | Control | 1093.1 ± 76.5 | 1174.4 ± 103.5 | 2633.0 ± 187.0 | 74.7 ± 28.2 | 104.4 ± 17.5 | 15.1 ± 0.4 |
| BsGFB04 | 1195.7 ± 277.2 | 1334.2 ± 151.4 | 2836.2 ± 383.7 | 69.4 ± 15.3 | 123.0 ± 21.0 | 16.5 ± 2.8 | |
| KsGRB10 | 1145.4 ± 81.5 | 1638.0 ± 295.7 | 3209.3 ± 262.4 | 151.3 ± 17.2 | 136.8 ± 5.2 | 16.7 ± 1.9 | |
| HT | Control | 735.0 ± 69.4b | 854.2 ± 69.5b | 2198.0 ± 57.6 | 88.2 ± 42.5 | 116.3 ± 22.3 | 18.5 ± 1.7 |
| BsGFB04 | 859.6 ± 35.3b | 1399.0 ± 158.0a | 2464.1 ± 289.0 | 65.0 ± 19.4 | 118.8 ± 15.4 | 12.3 ± 1.2 | |
| KsGRB10 | 1197.2 ± 38.8a | 1188.2 ± 120.0ab | 2889.4 ± 241.5 | 80.1 ± 25.5 | 121.4 ± 25.5 | 17.8 ± 2.4 | |
| Treatment effects | |||||||
| TEMP, df = 1 | 4.261° (.061) | 3.011 (.108) | 2.809 (.120) | .909 (.359) | .027 (.871) | .003 (.960) | |
| INOC, df = 2 | 2.059° (.065) | 3.456° (.065) | 2.725 (.106) | 1.815 (.205) | .489° (.0625) | 1.319 (.304) | |
| TEMP × INOC, df = 2 | 1.647 (.233) | 1.302 (.308) | .022 (.978) | 1.415 (.281) | .263 (.773) | 2.050 (.171) | |
| Contrasts | |||||||
| Control vs Inoculated | 2.180 (.159) | 6.179* (.024) | 3.146 (.095) | 0.165 (.690) | 0.992 (.334) | 0.312 (.584) | |
| Control vs BsGFB04 | 0.433 (.526) | 6.888* (.025) | 0.784° (.397) | 0.311 (.589) | 0.360 (.562) | 1.514 (.247) | |
| Control vs KsGRB10 | 6.422* (.030) | 4.110° (.070) | 6.824* (.026) | 1.232 (.293) | 1.113 (.316) | 0.064 (.805) | |
| BsGFB04 vs KsGRB10 | 0.897 (.366) | 0.054 (.821) | 1.681 (.224) | 4.239° (.067) | 0.239 (.636) | 1.784 (.211) | |
Data are means (± SE) of three replicates, and means with the same letter in the same column and temperature treatment are not significantly different at P = 0.05 according to Tukey’s HSD test. F-values from two-way ANOVA are given together with P-values in brackets. °, P < 0.1 (highlighted in italics); *P < 0.05; **P < 0.01; ***P < 0.001 (highlighted in bold). Contrast matrix was used to test the comparisons with inoculation treatments
Control control treatment, BsGFB04 Bacillus sp. GFB04 inoculation, KsGRB10 Klebsiella sp. GRB10 inoculation, HT heat stress treatment, TEMP temperature treatment, INOC endophyte inoculation treatment