FIG. 6.
Effect of recombinant wild-type NSP3 and the eIF4G-binding mutant of NSP3 on the translation of rotavirus-like, non-poly(A), and poly(A) mRNAs in vitro. Wild-type NSP3 (NSP34–313) (left) or NSP3 mutated in its eIF4G-binding domain (NSP34–290) (right) was added to RRL programmed with different mRNAs (same abbreviations as in Fig. 3). Fold stimulation is expressed by the ratio of F.Luc activity obtained in the presence of recombinant protein to the activity obtained in the absence of recombinant protein.