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. 2024 Jul 3;7:806. doi: 10.1038/s42003-024-06447-4

Fig. 2. Persistent multiple CF innervation of PCs in lobules 8-9 but not in lobules 1-4/5 of adolescent TARPγ2-GC-KO mice.

Fig. 2

a–d Sample CF-EPSC traces (a, c) and frequency distribution histograms of PCs in terms of the number of discrete CF-EPSC steps (b, d) for control (open columns) and TARPγ2-GC-KO (filled columns) mice aged P21–P40. Holding potentials for (a, c), − 10 mV. Scale bars for (a, c), 10 ms, and 0.5 nA. ***P < 0.001, Mann-Whitney U test. e−l Triple immunofluorescence labeling for the PC marker calbindin (blue or gray), anterogradely-labeled (DA488-positive) CFs (red), and the CF terminal marker VGluT2 (green) in lobule 8 (e−h) and lobule 4/5 (i−l) of a control (e, f1, f2, i, j1, j2) and a TARPγ2-GC-KO (g, h1, h2, k, l1, l2) mouse at 3 weeks of age. Boxed regions in e, g, i, and k are enlarged in f1 and f2, h1 and h2, j1 and j2, and l1 and l2, respectively. Arrows in h1 and h2 indicate anterogradely unlabeled (DA488-negative) VGluT2-positive CF terminals. Scale bars: e, 20 μm; f1, 5 μm.