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. 2024 Jul 3;15:5589. doi: 10.1038/s41467-024-49443-2

Fig. 4. Intranasal immunization of a low and a high dose of a trivalent vaccine (TVC-IV) induces a similar level of serum IgG and IgA.

Fig. 4

A Immunization schedule in IFNAR1−/− mice. IFNAR1−/− mice (n = 5 or 6) were immunized intranasally with a high (1.2 × 106 PFU) or a low (3 × 105 PFU) dose of TVC-IV (rMeV-B.1.617.2, rMuV-JL1-B.1.1.7, and rMuV-JL2-WA1), a high dose (1.2 × 106 PFU) of rMuV-JL2-WA1, or MMM vector, and boosted with the same dose 3 weeks later. At weeks 2, 5, and 7, serum was collected from each mouse and S-specific IgG titer was determined by ELISA using preS-6P protein of SARS-CoV-2 WA1 (B), B.1.1.7 (C), B.1.351 (D), or B.1.617.2 (E) as the coating antigen. In addition, sera at week 7 were used for the detection of serum IgA using preS-6P of SARS-CoV-2 WA1 as the coating antigen (F). IgG and IgA titers are the GMT of 5 or 6 mice (n = 5 or 6) ± SD. One-way ANOVA was used for statistical analyses. “ns” denotes no significant difference. Source data are provided in the Source Data file.