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. 2024 Jul 3;14:15335. doi: 10.1038/s41598-024-65320-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Microbial differences between patients with and without AL. PCO underlying a Bray–Curtis resemblance measurement at genus level displaying groups (A) and with bubble-plot and vector overlay representing the abundance of genera across the sample (B). (C) Comparison of the average relative abundance of bacteria in AS and AL across all timepoints. (D) ROC-analysis for the occurrence of AL based on the abundance of certain bacterial genera. (E) Violin-plot of the score-index applied in the ROC-analysis, with the best suited cut-off displayed as dashed line (95% CI). (F) Comparison of the genus Prevotella between patients with and without stoma. (G) Timepoint specific relative abundance of bacteria in AS and AL. Arrow displays statistically significant trend over time in one group (trendyspliner-Test). Bracket displays statistically significant differences between two groups over the full-time course (permuspliner-Test).