FIG. 3.

CLSM analysis of incoming Ad2-TR in cells treated with actin and PKC inhibitors reveals the actin and PKC requirements for nuclear transport of Ad2. TR-labeled Ad2 (red) was bound to HeLa cells pretreated with drugs or not pretreated and internalized for 0 min (A) or 60 min (B to E) as indicated in Materials and Methods. CLSM sections sampling the entire cell were generated for the TR channel and the Alexa 488 channel (plasma membrane Ca ATPase 1 [green]) and projected en face. The limits of the nucleus as determined by DAPI staining (results not shown) are indicated by a white trace. A single representative cell for each of the conditions is shown.