Fig. 1.

A) Expression and purification of E184L protein with MBP-tag. Bacterial lysates from E. coli. ER2523 with recombinant plasmids pMAL-C5x-E184L and purified E184L protein were subjected to SDS-PAGE (right) and Western blot analysis with anti-MBP mAb (left). Sample order in both SDS-PAGE and Western blot were as follows: lane 1, the supernatant of ER2523 with pMAL-C5x- E184L induced by IPTG after sonication; lane 2, the precipitation of ER2523 with pMAL-C5x- E184L; Lane 3, pMAL-C5x- E184L without IPTG addition; and lane 4, the purified E184L protein. B) Reactivity of E184L to ASFV-positive pig serum, lane 1 was loaded with MBP (negative control) and lane 2 was loaded with E184L protein and stained with ASFV-positive serum at a dilution of 1:1000.