Fig. 8.

Assessing the potential application of the two nove E184L epitopes. A) Schematic presentation of the E184L-dual epitope design. B) Purification of the E184L-dual epitope protein, lane 1 and lane 2 were loaded with 20 μL purified protein and lane 3 was loaded with 20 μL purified MBP protein (control). C) Reactivity test of the synthesized E184L-dual epitope protein to the four mAbs prepared in the study. Anti-E184L polyclonal mouse serum and SP2/0 cell supernantnts were used as positive and negative control, respectively. D) Dual epitope-based ELISA to confirm the antigenecity and potential application of the two novel E184L epitopes.