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. 2000 Aug;74(15):7108–7118. doi: 10.1128/jvi.74.15.7108-7118.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 3 — Replication-defective adenovirus vectors for expression of IE72 or IE86 protein. (A) Diagram of replication-defective adenovirus vectors. Ad-GFP expresses the humanized GFP. Ad-Trans expresses the TetR-VP16 fusion protein, which binds to the TRE and activates the minimal CMV promoter (P_minCMV). The Ad-IE72 and Ad-IE86 replication-defective adenovirus vectors express the IE72 or IE86 protein under control of the TRE-P_minCMV promoter. (B) Western blot analysis of IE72 and IE86 protein expression detected by monoclonal antibody 810. Lanes: 1, 3 to 6, and 8 to 10, HFF; 2 and 7, 293 cells; 1 and 6, no adenovirus vector treatment; 2, Ad-IE72 plus Ad-Trans; 3, Ad-IE72 alone; 4, Ad-IE72 plus Ad-Trans; 5, Ad-IE72 plus Ad-Trans in the presence of 10 μg of tetracycline/ml; 7, Ad-IE86 plus Ad-Trans; 8, Ad-IE86 alone; 9, Ad-IE86 plus Ad-Trans; 10, Ad-IE86 plus Ad-Trans in the presence of 10 μg of tetracycline/ml.