FIG. 5.

Effect of the IE86 protein on the S phase of permissive U373 cells. U373 cells, serum starved for 48 h, were treated with either 10 PFU of Ad-GFP/cell or of 5 PFU each of either Ad-IE72 plus Ad-IE86, Ad-IE72 plus Ad-Trans, or Ad-IE86 plus Ad-Trans per cell. After 24 h, the cells were treated with nocodazole and stimulated with high-serum medium. After an additional 24 h, the cells were stained with propidium iodide (PI), and the DNA content of an equal number of cells was determined using a FACS scanner. The PI profile is shown as the solid histogram line. The PI histogram cell cycle analysis was determined by use of the ModFit program as described in Materials and Methods and is given as percent G₀/G₁/percent S/percent G₂/M.