FIG. 6.

Expression of wild-type and mutant IE86 proteins. (A) Diagram of plasmids used for GFP, IE86, or IE86HL protein expression. An SV40 promoter drives expression of GFP in a direction opposite to that of the MIE promoter (MIEP) driving expression of either no viral gene (GFP), HCMV IE86 (GFP-IE86), or a mutant form of IE86 (GFP-IE86HL). The CRS of the MIE promoter was mutated (crs−) to allow equal expression of both the IE86 and IE86HL proteins. (B) Western blot analysis of IE86 and IE86HL protein expression detected by monoclonal antibody 810. The 293T cells were transiently transfected with 10 μg of either plasmid GFP, GFP-IE86, or GFP-IE86HL.