FIG. 5.

Immunoprecipitation of C-terminally truncated gB. Lysates from metabolically radiolabeled RK13 cells expressing wild-type gB (A, lane 1), gB-008 (A, lane 2), gB-007 (A, lane 3), gB-006 (A, lane 4), or gB-005 (A, lane 5) and from normal RK13 cells (A, lane 6) or RK13 cells infected with PrV-1112 (B, lane 1), PrV-ΔgBGFPR (B, lane 2), PrV-008 (B, lane 3), PrV-007 (B, lane 4), or PrV-ΔgBβ (B, lane 5) were precipitated with the gB-specific MAb a80-c16. Precipitates were analyzed by SDS-PAGE under reducing conditions, and labeled protein was visualized by autoradiography.