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. 2024 Jul 4;43:185. doi: 10.1186/s13046-024-03095-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — LOC101928222 promotes CRC progression by regulating HMGCS2 expression. A Subcellular localization of LOC101928222 in SW480 and LOVO cells. The nuclear control was U6, and the cytoplasm control was GAPDH, β-actin. B FISH analysis of the location of LOC101928222 in the cytoplasm and nuclear fractions of SW480 and LOVO cells. C Heatmap of differentially expressed mRNA identified by RNA-seq after LOC101928222 knockdown in LOVO cells. D Metascape website for enrichment analysis of differential genes. E qRT-PCR were performed to validate genes with high differential expression folds in the RNA-seq results. F Western blotting was used to detect changes in HMGCS2 protein levels after knockdown and overexpression of LOC101928222. G Tube formation rescue assays for effects of HMGCS2 overexpression on angiogenesis of SW480 and LOVO cells with LOC101928222 shRNA. Scale bar: 100 μm. H Transwell rescue assays for effects of HMGCS2 overexpression on migration and invasion of SW480 and LOVO cells with LOC101928222 shRNA. Scale bar: 50 μm.*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001