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. 2000 Aug;74(15):7146–7150. doi: 10.1128/jvi.74.15.7146-7150.2000

FIG. 1.

FIG. 1

Antibody-enhanced dengue virus infection of KU812 cells. (A) Cultures of Vero, P815, or KU812 cells were inoculated with dengue type 2 virus strain 16681 (19) (multiplicity of infection [MOI], 0.1), RSV (MOI, 0.1), or combinations of either virus with the respective human immune serum (final dilution, 1:1000). Virus infection was monitored by radiolabeling with [35S]methionine-cysteine, followed by immunoprecipitation and fluorographic sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The positions of dengue virus proteins (NS1)2, E, and prM and of RSV proteins N and M are indicated. (B) Vero cells (positive control) and KU812 cells were inoculated with dengue virus alone (Den) (MOI, 0.2), and normal human serum (NHS) (final dilution, 1:1,000) or dengue virus and human dengue virus immune sera (Ab; final dilutions of 1:1,000 or 1:10,000). The positions of the radiolabeled (NS1)2 and E proteins are indicated. Data are representative of nine separate experiments. Human sera, described in reference 20, were obtained from patients convalescing from dengue virus type 2 infection; normal human AB sera and RSV-positive sera were obtained from volunteer donors.