Fig. 5.

Circulating monocytes from adult hematopoiesis do not contribute to the resident hyalocyte pool under homeostasis. a Graphical scheme of parabiosis experiments. Ubc^GFP/+ donor mice were surgically connected to Ubc^+/+ wildtype mice for parabiosis and retinal whole mounts subsequently analyzed by fluorescence microscopy after 4 and 28 weeks, respectively. b Confocal images of retinal flat mounts from Ubc^+/+ acceptor parabionts. Hyalocytes (asterisks) and rMG (arrowheads) in the inner plexiform layer can be identified. Images are representative for three mice (4 weeks) and four mice (28 weeks), respectively. Scale bar = 50 µm. c Quantification of GFP⁺ cells among IBA1⁺ hyalocytes and rMG in Ubc^+/+ parabiotic mice 4 (N = 3) and 28 (N = 4) weeks after surgery. Each symbol represents one animal. Blood chimerism of CD11b⁺ myeloid blood cells in Ubc^+/+ recipient parabionts, as assessed by flow cytometry, was 46.93 ± 5.6% for 4 weeks and 52.98 ± 6.9% for 28 weeks post surgery. d Graphical illustration describing the experimental setup. In Flt3^Cre:Rosa26-YFP mice, constitutive activity of Cre-recombinase leads to YFP expression, under the control of the Rosa26-promoter, in all FLT3⁺ hematopoietic cells during fetal and postnatal hematopoiesis and their progeny. e Fluorescent microscopic visualization of IBA1 (red) and YFP (green) in hyalocytes (asterisks) and rMG (arrowheads) in Flt3^Cre:Rosa26-YFP mice. Images are representative for four mice. Scale bar = 100 µm. f Quantification of YFP⁺ cells among IBA1⁺ hyalocytes and rMG in Flt3^Cre:Rosa26-YFP mice (N = 4). Each symbol represents one animal from one litter