| Gene (Caenorhabditis elegans) |
myrf-1 |
WormBase Gene ID |
WBGene00004134 |
other name: pqn-47 |
| Gene (Caenorhabditis elegans) |
myrf-2 |
WormBase Gene ID |
WBGene00008999 |
|
| Gene (Caenorhabditis elegans) |
pan-1 |
WormBase Gene ID |
WBGene00003915 |
|
| Gene (Caenorhabditis elegans) |
lin-4
|
WormBase Gene ID |
WBGene00002993 |
|
| Gene (Caenorhabditis elegans) |
mir-48 |
WormBase Gene ID |
WBGene00003039 |
|
| Gene (Caenorhabditis elegans) |
mir-73 |
WormBase Gene ID |
WBGene00003301 |
|
| Gene (Caenorhabditis elegans) |
mir-230 |
WormBase Gene ID |
WBGene00003323 |
|
| Chemical compound, drug |
G418 |
BBI LIFE SCIENCES |
A600958-0001 |
25 mg/mL |
| Commercial assay or kit |
ClonExpress II One Step Cloning |
Vazyme Biotech Co., Ltd |
C112-01 |
|
| Commercial assay or kit |
FastPure Plasmid Mini Kit |
Vazyme Biotech Co., Ltd |
DC201-01 |
|
| Commercial assay or kit |
Taqman microRNA RT kit |
ThermoFisher |
4366596 |
|
| Commercial assay or kit |
TAKARA RNA reverse transcription kit |
TAKARA |
6110 A |
|
| Strain, strain background (Caenorhabditis elegans) |
lin-4(umn84[lin-4p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722])/mIn1[dpy-10(e128) umnIs33] II. |
Caenorhabditis Genetics Center |
CGC177 |
Figure 2C–H, Figure 2—figure supplement 2, Figure 2—figure supplement 3A–D, Figure 5A, B, E and F
|
| Strain, strain background (Caenorhabditis elegans) |
unc-119(ed3) III; lin-4p::GFP +unc-119(+)(maIs134). |
Caenorhabditis Genetics Center |
VT1072 |
Figure 2A–B, Figure 4A, Figure 5C–D, Figure 6A–D, Figure 2—figure supplement 1, Figure 6—figure supplement 1
|
| Strain, strain background (Caenorhabditis elegans) |
lin-14::GFP(cc2841) X. |
Caenorhabditis Genetics Center |
PD1301 |
Figure 3A–B
|
| Strain, strain background (Caenorhabditis elegans) |
GFP::pqn-47::3xFlag(ybq14) II; glo-1(zu391). |
This paper; The Yingchuan B. Qi Laboratory |
BLW1827 |
Figure 1A–B, Figure 6—figure supplement 2A–C
|
| Strain, strain background (Caenorhabditis elegans) |
myrf-1(ju1121) / mIn1 II; lin-4p::GFP +unc-119(+)(maIs134). |
This paper; The Yingchuan B. Qi Laboratory |
BLW1424 |
Figure 2A–B, Figure 4A
|
| Strain, strain background (Caenorhabditis elegans) |
myrf-1(ju1121) II / [mIs14 dpy-10(e128)](mIn1) II; lin-14::GFP(cc2841) X. |
This paper; The Yingchuan B. Qi Laboratory |
BLW1410 |
Figure 3A–B
|
| Strain, strain background (Caenorhabditis elegans) |
lin-4(umn84[lin-4p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722]) II myrf-1(ybq6) II /mIn1[dpy-10(e128) umnIs33] II. |
This paper; The Yingchuan B. Qi Laboratory |
BLW2284 |
Figure 2C–D, Figure 2—figure supplement 3A
|
| Strain, strain background (Caenorhabditis elegans) |
lin-4(umn84[lin-4p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722]) II myrf-1(ybq6) II /mIn1[dpy-10(e128) umnIs33] II; myrf-2(ybq42) X. |
This paper; The Yingchuan B. Qi Laboratory |
BW2285 |
Figure 2C–D, Figure 2—figure supplement 3A
|
| Strain, strain background (Caenorhabditis elegans) |
lin-4 umn84[lin-4p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722 / dpy-10(e128)(mT1) II, III; pan-1(gk142) III / dpy-10(e128), Pmyo-2-mCherry_chr_2(mT1-mCherry#2) III. |
This paper; The Yingchuan B. Qi Laboratory |
BLW2300 |
Figure 2G–H, Figure 2—figure supplement 3B
|
| Strain, strain background (Caenorhabditis elegans) |
lin-4(umn84[lin-4p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722]) II /mIn1[dpy-10(e128) umnIs33] II; myrf-2(ybq42) X. |
This paper; The Yingchuan B. Qi Laboratory |
BLW2385 |
Figure 2C–D, Figure 2—figure supplement 3A
|
| Strain, strain background (Caenorhabditis elegans) |
lin-4(umn84[lin-4p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722]) II myrf-1(ju1121) II /mIn1[dpy-10(e128) umnIs33] II. |
This paper; The Yingchuan B. Qi Laboratory |
BLW2236 |
Figure 2E–F, Figure 2—figure supplement 3C–D
|
| Strain, strain background (Caenorhabditis elegans) |
lin-4(umn84[lin-4p::SL1::EGL-13NLS::lox2272::mScarlet-I::cMycNLS::Lox511I::let-858 3'UTR::lox2722]) II myrf-1(ybq1313) II /mIn1[dpy-10(e128) umnIs33] II. |
This paper; The Yingchuan B. Qi Laboratory |
BLW2237 |
Figure 5E–F
|
| Strain, strain background (Caenorhabditis elegans) |
myrf-1(ju1121) II / [mIs14 dpy-10(e128)](mIn1) II; lin-4p::GFP +unc-119(+)(maIs134); Pmyo-3-MYRF-1(ybqEx746). |
This paper; The Yingchuan B. Qi Laboratory |
BLW1579 |
Figure 4A
|
| Strain, strain background (Caenorhabditis elegans) |
myrf-1(ju1121) II / [mIs14 dpy-10(e128)](mIn1) II; lin-4p::GFP +unc-119(+)(maIs134); Pdpy-7-GFP::MYRF-1(ybqEx721). |
This paper; The Yingchuan B. Qi Laboratory |
BLW1580 |
Figure 4A
|
| Strain, strain background (Caenorhabditis elegans) |
gfp::myrf-1(LoxP) (ybq98) II. |
This paper; The Yingchuan B. Qi Laboratory |
BLW1934 |
Figure 4B–E
|
| Strain, strain background (Caenorhabditis elegans) |
gfp::myrf-1(LoxP)(ybq98) / [mIs14 dpy-10(e128)](mIn1) II; Pdpy-7-NLS::Cre(tmIs1028); lin-4p::GFP +unc-119(+)(maIs134). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2020 |
Figure 4B–E
|
| Strain, strain background (Caenorhabditis elegans) |
GFP::MYRF-1 (1–700)::3xFlag(syb1313) II / [mIs14 dpy-10(e128)](mIn1) II; lin-4p::GFP +unc-119(+)(maIs134). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2037 |
Figure 5C–D
|
| Strain, strain background (Caenorhabditis elegans) |
Ppqn-47-LoxP-NLS::tagRFP::T2A::pqn-47-LoxP(ybqIs112) V. |
This paper; The Yingchuan B. Qi Laboratory |
BLW1235 |
Figure 6A
|
| Strain, strain background (Caenorhabditis elegans) |
lin-4p::GFP +unc-119(+)(maIs134); Ppqn-47-LoxP-NLS::tagRFP::T2A::pqn-47-LoxP(ybqIs112) V. |
This paper; The Yingchuan B. Qi Laboratory |
BLW2172 |
Figure 6A–C, Figure 6—figure supplement 1A–B
|
| Strain, strain background (Caenorhabditis elegans) |
GFP::pqn-47::3xFlag(ybq14) II, glo-1(zu391), Prpl-28-tetR-tagRFP (ybqSi233). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2170 |
Figure 6G–I
|
| Strain, strain background (Caenorhabditis elegans) |
Prpl-28-tetR-tagRFP (ybqSi233). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2168 |
Cross with BLW1827 |
| Strain, strain background (Caenorhabditis elegans) |
Pmir-48-GFP(ybqSi206). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2113 |
Figure 7C–D
|
| Strain, strain background (Caenorhabditis elegans) |
Pmir-73-GFP(ybqSi208). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2115 |
Figure 7C–D
|
| Strain, strain background (Caenorhabditis elegans) |
Pmir-230-GFP(ybqSi209). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2116 |
Figure 7C–D
|
| Strain, strain background (Caenorhabditis elegans) |
myrf-1(ju1121) II / [mIs14 dpy-10(e128)](mIn1) II, Pmir-48-GFP(ybqSi206). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2181 |
Figure 7C–D
|
| Strain, strain background (Caenorhabditis elegans) |
myrf-1(ju1121) II / [mIs14 dpy-10(e128)](mIn1) II, Pmir-73-GFP(ybqSi208). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2182 |
Figure 7C–D
|
| Strain, strain background (Caenorhabditis elegans) |
myrf-1(ju1121) II / [mIs14 dpy-10(e128)](mIn1) II, Pmir-230-GFP(ybqSi209). |
This paper; The Yingchuan B. Qi Laboratory |
BLW2183 |
Figure 7C–D
|
| Strain, strain background (Caenorhabditis elegans) |
gfp::myrf-1 (1–656)(syb1468) II / [mIs14 dpy-10(e128)](mIn1) II; lin-4p::GFP +unc-119(+)(maIs134) |
This paper; The Yingchuan B. Qi Laboratory |
BLW2184 |
Figure 2—figure supplement 1A–B
|
| Strain, strain background (Caenorhabditis elegans) |
gfp::myrf-1 (1–482)(syb1491) II / [mIs14 dpy-10(e128)](mIn1) II; lin-4p::GFP +unc-119(+)(maIs134) |
This paper; The Yingchuan B. Qi Laboratory |
BLW2035 |
Figure 2—figure supplement 1A–B
|
| Strain, strain background (Caenorhabditis elegans) |
gfp::degron::myrf-1::3xflag(ybq133) II; eft-3p::TIR::F2A:mTagBFP2::AID*::NLS::tbb-2 3'UTR(wrdSi23) I; lin-4p::GFP +unc-119(+)(maIs134) |
This paper; The Yingchuan B. Qi Laboratory |
BLW2157 |
Figure 2—figure supplement 4A–B
|
| Recombinant DNA reagent |
Pmyo-3-MYRF-1 |
This paper; The Yingchuan B. Qi Laboratory |
pQA1094 |
Figure 4A
|
| Recombinant DNA reagent |
Pdpy-7-MYRF |
This paper; The Yingchuan B. Qi Laboratory |
pQA1511 |
Figure 4A
|
| Recombinant DNA reagent |
Peft-3-Cas9 U6-myrf-1 sgRNA (for cut MYRF-1 211 K) |
This paper; The Yingchuan B. Qi Laboratory |
pQA1685 |
sgRNA plasmid for constructing BLW1934 [gfp::myrf-1(LoxP) (ybq98)] |
| Recombinant DNA reagent |
Peft-3-Cas9 U6-myrf-1 sgRNA (for cut MYRF-1 201 V) |
This paper; The Yingchuan B. Qi Laboratory |
pQA1686 |
sgRNA plasmid for constructing BLW1934 [gfp::myrf-1(LoxP) (ybq98)] |
| Recombinant DNA reagent |
myrf-1 LoxP knock-in repair template in pCR8 |
This paper; The Yingchuan B. Qi Laboratory |
pQA1688 |
repair template plasmid for constructing BLW1934 [gfp::myrf-1(LoxP) (ybq98)] |
| Recombinant DNA reagent |
Prpl-28-GFP-myrf-1 (1–931 delete 601–650 Ce) |
This paper; The Yingchuan B. Qi Laboratory |
pQA1922 |
Figure 5B
|
| Recombinant DNA reagent |
Prpl-28-tetR-tagRFP in_miniMos |
This paper; The Yingchuan B. Qi Laboratory |
pQA1896 |
plasmid for constructing BLW2168 [Prpl-28-tetR-tagRFP (ybqSi233)] |
| Recombinant DNA reagent |
7x-TetO |
This paper; The Yingchuan B. Qi Laboratory |
pQA1961 |
Figure 6G–I
|
| Recombinant DNA reagent |
Plin-4(2412 bp) -unc-54–3'UTR-7x-TetO |
This paper; The Yingchuan B. Qi Laboratory |
pQA1960 |
Figure 6G–I
|
| Recombinant DNA reagent |
Plin-4(2412 bp) -unc-54–3'UTR |
This paper; The Yingchuan B. Qi Laboratory |
pQA1881 |
Figure 6—figure supplement 2A–C
|
| Recombinant DNA reagent |
Pmir-48(2000 bp)-GFP in_miniMos |
This paper; The Yingchuan B. Qi Laboratory |
pQA1861 |
plasmid for constructing BLW2114 |
| Recombinant DNA reagent |
Pmir-73(2000 bp)-GFP in_miniMos |
This paper; The Yingchuan B. Qi Laboratory |
pQA1863 |
plasmid for constructing BLW2115 |
| Recombinant DNA reagent |
Pmir-230(1919 bp)-GFP in_miniMos |
This paper; The Yingchuan B. Qi Laboratory |
pQA1864 |
plasmid for constructing BLW2116 |
| Recombinant DNA reagent |
pGH8 Prab-3::mCherry |
The Erik Jorgensen Laboratory |
pGH8 |
Co-marker plasmid for constructing single-copy integrated strain |
| Recombinant DNA reagent |
pCFJ90 Pmyo-2::mCherry |
The Erik Jorgensen Laboratory |
pCFJ90 |
Co-marker plasmid, Figure 6—figure supplement 2A–C
|
| Recombinant DNA reagent |
pCFJ601 Peft-3 Mos1 transposase |
The Erik Jorgensen Laboratory |
pCFJ601 |
Co-marker plasmid for constructing single-copy integrated strain |
| Recombinant DNA reagent |
PDD162 Peft-3-Cas9 & Empty sgRNA |
The Bob Goldstein Laboratory |
PDD162 |
sgRNA plasmid template |
