TABLE 2.
Effect of PBMC and IL-15 neutralization on HSV-1 replicationa
| Treatment | HSV-1 titer
|
||
|---|---|---|---|
| K562 | K562 + PBMC I | K562 + PBMC II | |
| Anti-IL-15 antibodies | 4.7 × 105 | 3.43 × 104 | 4.83 × 104 |
| Control antibodies | 3.50 × 105 | 2.17 × 103 | 2.46 × 103 |
| rhIL-15 | 6.10 × 105 | 6.2 × 102 | 9.5 × 102 |
a
K562 cells (105) were infected with HSV-1 (106 PFU), washed, and cocultured with mock-treated (PBMC I) or UV-inactivated HSV-1-treated (PBMC II) cells (5 × 106) with or without the presence of IL-15-neutralizing antibodies or of rhIL-15 (100 ng/ml). On day 3 postinfection, HSV-1 titers in these cultures were determined; the results of a typical experiment are shown.