Extended Data Fig. 3. Genotype, Environment and Infection interactions determine immune composition in murine MLNs.
(A) PCA of immune cell clusters identified by unsupervised clustering in the MLN with the myeloid panel. (n = 73; 21 129S1, 30 C57BL/6 and 22 PWK/PhJ mice) (B) PCA of immune cell clusters identified by unsupervised clustering in the MLN (n = 73; 21 129S1, 30 C57BL/6 and 22 PWK/PhJ mice) with the lymphoid panel reflecting PC1 and PC3 with (C) Box plot showing the variance on PC3 axis of PCA plots. The box plot center line represents median, the boundaries represent IQR with the whiskers representing the upper and lower quartiles ±1.5 Interquartile Range (IQR), all individual data points are shown (129S1 Uninfected = 9, C57BL/6 Uninfected= 16, PWK-PhJ Lab Uninfected = 7, 129S1 T. muris = 12, C57BL/6 T. muris = 15, PWK-PhJ T. muris = 15). Statistical significance was determined by one-way ANOVA one tailed test between different groups of interest with Graph-Pad Software (C).