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. 2024 Jun 14;25(7):1270–1282. doi: 10.1038/s41590-024-01862-5

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 5 — MLN cells from different strains of mice in the laboratory and rewilded conditions were harvested and the proportion of the Naïve and memory CD4 and CD8 T cell subset were determined by flow cytometric analysis. Representative flow cytometry plots and quantification of CD62L^hiCD44^lo, CD62L^hiCD44^hi, and CD62L^loCD44^hi CD4 T cells in CD4 T cells (A), (B) and the CD8 T cell (C) and (D) cellular population. For (B) and (D), n = 117; 129S1 Lab Uninfected = 8, 129S1 Lab T. muris = 9, 129S1 RW Uninfected = 8, 129S1 RW T. muris = 12, C57BL/6 Lab Uninfected = 6, C57BL/6 Lab T. muris = 9, C57BL/6 RW Uninfected = 17, C57BL/6 RW T. muris = 15, PWK/PhJ Lab Uninfected = 7, PWK/PhJ Lab T. muris = 5 PWK/PhJ RW Uninfected = 6, PWK/PhJ RW T. muris = 15 over two experimental blocks. Grubb’s outlier test was done to remove outliers. Bar plots showing the pseudo R² measure of effect size of predictor variables and interactions as calculated by multivariate distance matrix regression analysis (MDMR) in based on (E) proportions and (F) cell numbers from the CD4 and CD8 T cell lymphoid cells CD4 T cell clusters harvested from the MLN. Raw data – Data File S6.

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