Extended Data Fig. 1. Unsupervised Clustering of murine PBMC cells.
(A) PCA Plot showing clusters generated following unsupervised clustering of PBMCs cells (n = 64; 17 129S1, 29 C57BL/6 and 18 PWK/PhJ mice) (B) Bar plot showing cluster percentage in different groups on a per mice basis. (C) the loading factors of immune clusters for PCA plot of PBMC cells showing PC1 and PC2 axis (D) PCA showing PC1 and PC3 axis of immune cell clusters identified by unsupervised clustering in the PBMCs cells and (E) Box plot showing variance on PC3 axis of PCA plots in (D). The box plot center line represents median, the boundaries represent IQR with the whiskers representing the upper and lower quartiles ±1.5 Interquartile Range (IQR), all individual data points are shown (129S1 Uninf = 8, C57BL/6 = 14, PWK-PhJ Lab = 6, 129S1 RW = 9, C57BL/6 RW = 15, PWK RW = 12). Statistical significance in (E) was determined by one-way ANOVA test between different groups with Graph-Pad Software. ns p > 0.05.