Fig. 1. Kinetics of immune infiltration correlate with pain development.

a, Heat hypersensitivity in inflamed paws measured by the latency to react in the Hargreaves assay before and 4, 24 and 48 h after zymosan injection (n = 9, male 4, female 5), incision (n = 9, male 4, female 5), UV burn (n = 14, male 5, female 9) in the paws of wild-type (WT) mice. Data are represented as mean value ± s.e.m. P values calculated using one-way ANOVA, Tukey’s multiple comparison test; 8–12-week-old mice were used. b, t-SNE plot of scRNA-seq data of hematopoietic CD45⁺ cells-enriched skin from WT mice integrated from all samples. This comprises zymosan injection, incision and UV burn at 4 h, 24 h and 48 h post-injury and control skin from the CL paws at 4 h (zymosan), 24 h (incision) and 48 h (UV burn). c, Stacked area plot of mean proportions of immune cell types at 4 h, 24 h and 48 h in zymosan injection, incision and UV burn and CL healthy skin as in b. Ccr2⁻ recMacs, Ccr2⁺ recMacs and neutrophils were significantly changed following injury and are marked with an asterisk. d, Proportions of Ccr2⁻ recMacs, Ccr2⁺ recMacs and neutrophils at 4 h, 24 h and 48 h in zymosan, incision and UV burn injury. *Significant change in cell proportions compared to CL based on scCODA analysis. n.s., no statistically significant change in proportions of the cell type based on scCODA analysis (Methods).