FIG. 1.

Detection of STAT-1 by Western blotting and MMPs by gelatin zymography. (A) Western blot analysis of serial dilutions of protein from HIV-infected human macrophages detected both of the STAT-1 isoforms, STAT-1α and STAT-1β. Protein abundance was assessed by densitometry and equalized to the corresponding level of housekeeping protein detected in each sample. Similar results were obtained using FIV-infected feline brain and macrophages. Data are expressed as pixels per square inch (ppi) and represent the mean ± standard deviation (SD) of two experiments. (B) MMP-2 and -9 abundance was measured by gelatin zymography using conditioned medium from HIV-infected human (shown) and FIV-infected feline macrophages. Coomassie-stained gels were converted to grey-scale, and protein levels were quantified by densitometry and equalized to the number of cells in each sample well at the time of harvest. Data represent the mean ± standard deviation (SD) of three experiments.