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Workflow of proteomic sample preparation. (A) Frogs were treated under different conditions: control, anoxic, dehydrated, and frozen exposures. Liver tissues were sampled after the treatment and used for proteomic analysis. (B) Proteomic sample preparation was performed using the FASP method. Extracted proteins from frog liver tissues were reduced, alkylated, and digested into peptides and then analyzed with nLC-MS/MS.
