Fig. 3. H2A.Z deletion and expression of lysine mutants impact gene expression.

Volcano plots showing effects of H2A.Z deletion alone (H2A.Z^KO) and H2A.Z deletion combined with H2A.Z.1^WT, H2A.Z.1^KA, or H2A.Z.1^KQ replacement on transcript levels in (A) female and (B) male mice. Red and green circles indicate differentially expressed genes (DEGs) with FDR < 0.05 and log₂FC > 1 that are significantly increased or decreased relative to the Myc control group. Gray circles represent significant DEGs with FDR < 0.05 and log₂FC < 1. Black circles indicate genes that did not meet significance criteria. Number of DEGs shown in brackets reflect FDR < 0.05 and log₂FC > 1. Example gene tracks are shown for (C) female and (D) male mice. Data in gene tracks are expressed as Counts Per Million (CPM). N/group is as follows. Males: Myc 4, H2A.Z^KO 4, H2A.Z.1^WT5, H2A.Z.1^KA 4, H2A.Z.1^KQ 5. Females: Myc 3, H2A.Z.1^KO4, H2A.Z.1^WT 4, H2A.Z.1^KA 4, H2A.Z.1^KQ 3.