FIG. 3.

Analysis of viral protein synthesis in COS-1 cells transfected with integrin subunit cDNAs. Cells were transfected with plasmids encoding human or bovine integrin subunits as shown. Transfected cells were infected with FMDV type A₁₂, vRM-SSP, or vCRM8 at an MOI of 10 PFU/cell. Cells were labeled with [³⁵S]methionine, RIP was performed on cell lysates, and the products were analyzed by SDS-PAGE (10% polyacrylamide). Locations of viral structural proteins are denoted on the left. con, nontransfected cells; M, marker viral proteins from FMDV-infected BHK-21 cells also labeled with [³⁵S]methionine; bov, bovine integrin subunits; hum, human integrin subunits.