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. 2024 Jul 5;57:44. doi: 10.1186/s40659-024-00517-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 16 — a Amplification and melt peak plot of Bax and Beta actin genes in buck spermatozoa treated with different concentrations (0.5, 5, 10 and 20 ppm) of lead acetate after 15 min and 3 h. b Fold change in expression of Bax gene in spermatozoa following in-vitro exposure to different concentrations of lead acetate for different time intervals. Data presented are mean ± SE of the semen samples of six bucks. Different capital superscripts on bars indicate significant (p < 0.05) differences between the different time intervals while small superscripts on bars indicate significant (p < 0.05) differences between different concentration of lead