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A lytic replication cycle can be induced from the HVS stably transduced A549 cell line. HVS-infected A549 cells were incubated in the presence of either no addition (lane 1), 20 ng of TPA per ml (lane 2), or 3 mM n-butyrate (lane 3) for 48 h. After chemical induction, virus recovery assays were performed. The numbers of plaques formed are shown in graphical format: the variations between three replicate assays are indicated.
