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. 2000 Aug;74(16):7331–7337. doi: 10.1128/jvi.74.16.7331-7337.2000

FIG. 3.

FIG. 3

(a) Transcription mapping of the HVS genome in latently infected A549 cells. Total RNA was extracted from A549 cells (lane 1), HVS-stably transduced A549 cells (lane 2), OMK cells (lane 3), 16-h-infected OMK cells (lane 4), and 24-h-infected OMK cells (lane 5) and then separated by gel electrophoresis on a 1% denaturing formaldehyde-agarose gel. The RNA was transferred to Hybond-N membranes and hybridized with radiolabeled probes specific for EcoJ (i) and KpnE (ii). (b) Total RNA was extracted from A549 cells (lane 1) and HVS-stably transduced A549 cells (lane 2) and separated by gel electrophoresis on a 1% denaturing formaldehyde-agarose gel. The RNA was transferred to Hybond-N membranes and hybridized with radiolabeled probes specific for ORF 71 (i), ORF 72 (ii), and ORF 73 (iii).