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. 2024 Jul 5;23:137. doi: 10.1186/s12943-024-02049-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Tumor cell-intrinsic MELK inhibition affects the infiltration and polarization of TAMs. (A) The effect of MELK knockdown on tumor growth in mouse xenograft models (n = 5/group). (B-C) The effect of MELK inhibition on tumor weight (B) and tumor volume (C) (n = 5/group). (D-G) IF staining reflecting the expression changes of PCNA (D), F4/80 (E), CD206 (F) and CD86 (G) upon MELK inhibition in tumor tissues. (H-I) Antibody filter array implying the effects of MELK expression on cytokines related to immune cell recruitment. (J) ELISA showing the effect of tumoral MELK inhibition on serum CCL2 concentration. (K) qRT‒PCR analysis detecting the expression change of CCL2 upon MELK inhibition. (L) The correlation between tumor weight and serum CCL2 concentration. (M) FCM analysis showing the role of MELK expression in CD8 + T-cell recruitment to tumor tissue. (N) FCM analysis showing the effect of MELK expression on the GZMA-positive cell proportion in recruited CD8 + T cells in tumor tissue. * p < 0.05, ** p < 0.01, *** p < 0.001