Fig. 2.

Biofilm of S. epidermidis after fixation by heat and crystal violet (blue color) staining at different periods of biofilm life cycle on two surfaces. Borosilicate Pyrex (A) and Polystyrene (B). The stain was added for 10 min to the biofilm then rinsed with distilled water. The remained blue stain reveals the biofilm that will be visualized by the naked eye. Detachment was noticeable on day 4 (decrease in blue color stain) on both supports and biofilm regeneration on day 7 (re-increase in the stained area in terms of blue color). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)