Fig. 5.

Fold change 2^-ΔΔct expressed in Log 10 for biofilm genes (a–i) after normalization with reference gene16srRNA compared to planktonic sample during seven days. Where ΔΔCt = ΔCt (sample) -ΔCt (control), the control is the planktonic sample. Some genes in biofilm were enhanced while others were significantly decreased as compared to planktonic. Fold change (log10) = 0 indicate same expression of the gene in biofilm and planktonic sample. Folds >0 indicate positive increase in the expression relative to the control, folds <0 indicate negative expression (downregulation) of the gene relative to control. The assay was performed with at least three independent sample. Analysis of variance was not performed; error bars indicate standard deviation of the means.